Nucleic Acids Research Advance Access originally published online on December 12, 2006
Nucleic Acids Research 2007 35(1):269-278; doi:10.1093/nar/gkl1023
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Nucleic Acids Research, 2007, Vol. 35, No. 1 269-278
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Molecular Biology |
Transcription factor distribution in Escherichia coli: studies with FNR protein
School of Biosciences, University of Birmingham Edgbaston, Birmingham B15 2TT, UK 1 Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University Chikusa-ku, Nagoya 464-8601, Japan 2 Oxford Gene Technology, Begbroke Science Park Sandy Lane, Yarnton, Oxford OX5 1PF, UK
*To whom correspondence should be addressed. Tel: +44 121 414 5435; Fax: +44 121 414 7366; Email: d.grainger{at}bham.ac.uk
Received August 25, 2006. Revised November 8, 2006. Accepted November 14, 2006.
Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5' end of a gene, were identified. Some targets are adjacent to poorly transcribed genes where FNR has little impact on transcription. In stationary phase, the distribution of FNR was largely unchanged. Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution.
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