Nucleic Acids Research Advance Access originally published online on May 7, 2007
Nucleic Acids Research 2007 35(11):3590-3601; doi:10.1093/nar/gkm058
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Nucleic Acids Research, 2007, Vol. 35, No. 11 3590-3601
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Molecular Biology |
Redundant role of DEAD box proteins p68 (Ddx5) and p72/p82 (Ddx17) in ribosome biogenesis and cell proliferation
FR 2.3 Medical Biochemistry and Molecular Biology, Theoretical Medicine, University of the Saarland, D-66421 Homburg, Germany
*To whom correspondence should be addressed. Tel: +49 6841 16 26020; Fax: +49 6841 16 26521; Email: bchsta{at}uniklinikum-saarland.de
Received September 28, 2006. Revised November 12, 2006. Accepted January 18, 2007.
The DEAD box proteins encoded by the genes ddx5 (p68) and ddx17 (isoforms p72 and p82) are more closely related to each other than to any other member of their family. We found that p68 negatively controls p72/p82 gene expression but not vice versa. Knocking down of either gene does not affect cell proliferation, in case of p68 suppression, however, only on condition that p72/p82 overexpression was granted. In contrast, co-silencing of both genes causes perturbation of nucleolar structure and cell death. In mutant studies, the apparently redundant role(s) of p68 and p72/p82 correspond to their ability to catalyze RNA rearrangement rather than RNA unwinding reactions. In search for possible physiological targets of this RNA rearrangement activity it is shown that the nucleolytic cleavage of 32S pre-rRNA is reduced after p68 subfamily knock-down, most probably due to a failure in the structural rearrangement process within the pre-60S ribosomal subunit preceding the processing of 32S pre-rRNA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A.-M. J. Cziko, C. T. McCann, I. C. Howlett, S. A. Barbee, R. P. Duncan, R. Luedemann, D. Zarnescu, K. E. Zinsmaier, R. R. Parker, and M. Ramaswami Genetic Modifiers of dFMR1 Encode RNA Granule Components in Drosophila Genetics, August 1, 2009; 182(4): 1051 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Clark, A. Coulson, C. Dalgliesh, P. Rajan, S. M. Nicol, S. Fleming, R. Heer, L. Gaughan, H. Y. Leung, D. J. Elliott, et al. The RNA Helicase p68 Is a Novel Androgen Receptor Coactivator Involved in Splicing and Is Overexpressed in Prostate Cancer Cancer Res., October 1, 2008; 68(19): 7938 - 7946. [Abstract] [Full Text] [PDF]
|
|