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Nucleic Acids Research Advance Access originally published online on June 13, 2007
Nucleic Acids Research 2007 35(12):4203-4213; doi:10.1093/nar/gkm388
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Nucleic Acids Research, 2007, Vol. 35, No. 12 4203-4213
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis

Iván V. Rosado1, Dieter Kressler2 and Jesús de la Cruz1,*

1Departamento de Genética, Universidad de Sevilla, Sevilla, Spain and 2Biochemie-Zentrum der Universität Heidelberg, Heidelberg, Germany

*To whom correspondence should be addressed. Tel: +34 95 455 71 06; Fax: +34 95 455 71 04; Email: jdlcd{at}us.es

Received March 15, 2007. Revised April 30, 2007. Accepted April 30, 2007.

Ribosome synthesis in eukaryotes requires a multitude of trans-acting factors. These factors act at many steps as the pre-ribosomal particles travel from the nucleolus to the cytoplasm. In contrast to the well-studied trans-acting factors, little is known about the contribution of the ribosomal proteins to ribosome biogenesis. Herein, we have analysed the role of ribosomal protein Rpl3p in 60S ribosomal subunit biogenesis. In vivo depletion of Rpl3p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability of early and intermediate pre-ribosomal particles, as evidenced by the low steady-state levels of 27SA3, 27SBS and 7SL/S precursors. Furthermore, depletion of Rpl3p impairs the nucleocytoplasmic export of pre-60S ribosomal particles. Interestingly, flow cytometry analysis indicates that Rpl3p-depleted cells arrest in the G1 phase. Altogether, we suggest that upon depletion of Rpl3p, early assembly of 60S ribosomal subunits is aborted and subsequent steps during their maturation and export prevented.


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