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Nucleic Acids Research Advance Access originally published online on July 18, 2007
Nucleic Acids Research 2007 35(15):4989-5000; doi:10.1093/nar/gkm523
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Nucleic Acids Research, 2007, Vol. 35, No. 15 4989-5000
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Ctf18 is required for homologous recombination-mediated double-strand break repair

Hideaki Ogiwara1, Takashi Ohuchi1, Ayako Ui1, Shusuke Tada1, Takemi Enomoto1,2 and Masayuki Seki1,*

1Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578 and 2Tohoku University 21st Century COE Program "Comprehensive Research and Education Center for Planning of Drug development and Clinical Evaluation", Sendai, Miyagi 980-88578, Japan

*To whom correspondence should be addressed. Tel: +81 22 795 6875; Fax: +81 22 795 6873; Email: seki{at}mail.pharm.tohoku.ac.jp

Received March 10, 2007. Revised June 21, 2007. Accepted June 22, 2007.

The efficient repair of double-strand breaks (DSBs) is crucial in maintaining genomic integrity. Sister chromatid cohesion is important for not only faithful chromosome segregation but also for proper DSB repair. During DSB repair, the Smc1–Smc3 cohesin complex is loaded onto chromatin around the DSB to support recombination-mediated DSB repair. In this study, we investigated whether Ctf18, a factor implicated in the establishment of sister chromatid cohesion, is involved in DSB repair in budding yeast. Ctf18 was recruited to HO-endonuclease induced DSB sites in an Mre11-dependent manner and to damaged chromatin in G2/M phase-arrested cells. The ctf18 mutant cells showed high sensitivity to DSB-inducible genotoxic agents and defects in DSB repair, as well as defects in damage-induced recombination between sister chromatids and between homologous chromosomes. These results suggest that Ctf18 is involved in damage-induced homologous recombination.


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