Skip Navigation


Nucleic Acids Research Advance Access originally published online on August 28, 2007
Nucleic Acids Research 2007 35(17):e114; doi:10.1093/nar/gkm640
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1066K) Freely available
Right arrow Screen PDF (440K) Freely available
Right arrow Supplementary Material
Right arrowOA All Versions of this Article:
35/17/e114    most recent
gkm640v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chateigner-Boutin, A.-L.
Right arrow Articles by Small, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chateigner-Boutin, A.-L.
Right arrow Articles by Small, I.
Related Collections
Right arrow Nucleic acid modification
Right arrow RNA characterisation and manipulation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2007, Vol. 35, No. 17 e114
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons

Anne-Laure Chateigner-Boutin and Ian Small*

ARC Centre of Excellence for Plant Energy Biology, 4th Floor MCS Building, M316, University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia

*To whom correspondence should be addressed. Email: iansmall{at}cyllene.uwa.edu.au

Received July 1, 2007. Revised August 1, 2007. Accepted August 1, 2007.

We describe a rapid, high-throughput method to scan for new RNA editing sites. This method is adapted from high-resolution melting (HRM) analysis of amplicons, a technique used in clinical research to detect mutations in genomes. The assay was validated by the discovery of six new editing sites in different chloroplast transcripts of Arabidopsis thaliana. A screen of a collection of mutants uncovered a mutant defective for editing of one of the newly discovered sites. We successfully adapted the technique to quantify editing of partially edited sites in different individuals or different tissues. This new method will be easily applicable to RNA from any organism and should greatly accelerate the study of the role of RNA editing in physiological processes as diverse as plant development or human health.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DNA ResHome page
K. Yura, Y. Miyata, T. Arikawa, M. Higuchi, and M. Sugita
Characteristics and Prediction of RNA Editing Sites in Transcripts of the Moss Takakia lepidozioides Chloroplast
DNA Res, July 23, 2008; (2008) dsn016v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
N. O'Toole, M. Hattori, C. Andres, K. Iida, C. Lurin, C. Schmitz-Linneweber, M. Sugita, and I. Small
On the Expansion of the Pentatricopeptide Repeat Gene Family in Plants
Mol. Biol. Evol., June 1, 2008; 25(6): 1120 - 1128.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.