Skip Navigation


Nucleic Acids Research Advance Access originally published online on September 12, 2007
Nucleic Acids Research 2007 35(18):6181-6195; doi:10.1093/nar/gkm670
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1701K) Freely available
Right arrow Screen PDF (713K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
35/18/6181    most recent
gkm670v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ganguly, M.
Right arrow Articles by Gold, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ganguly, M.
Right arrow Articles by Gold, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2007, Vol. 35, No. 18 6181-6195
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Structural Biology

A study of 7-deaza-2'-deoxyguanosine–2'-deoxycytidine base pairing in DNA

Manjori Ganguly1,2, Feng Wang3, Mahima Kaushik2, Michael P. Stone3, Luis A. Marky1,2 and Barry Gold1,2,4,*

1Eppley Institute for Research in Cancer, 2Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE-68198-6025, 3Department of Chemistry, Vanderbilt University, Nashville, TN-37235 and 4Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA-15261, USA

*To whom correspondence should be addressed. Tel: +1 412 383 9593; Fax: +1 412 383 7436; Email: goldbi{at}pitt.edu

Received May 22, 2007. Revised July 26, 2007. Accepted August 13, 2007.

The incorporation of 7-deazaguanine modifications into DNA is frequently used to probe protein recognition of H-bonding information in the major groove of DNA. While it is generally assumed that 7-deazaguanine forms a normal Watson–Crick base pair with cytosine, detailed thermodynamic and structural analyses of this modification have not been reported. The replacement of the 7-N atom on guanine with a C–H, alters the electronic properties of the heterocycle and eliminates a major groove cation-binding site that could affect the organization of salts and water in the major groove. We report herein the characterization of synthetic DNA oligomers containing 7-deazaguanine using a variety of complementary approaches: UV thermal melting, differential scanning calorimetry (DSC), circular dichroism (CD), chemical probing and NMR. The results indicate that the incorporation of a 7-deazaguanine modification has a significant effect on the dynamic structure of the DNA at the flanking residue. This appears to be mediated by changes in hydration and cation organization.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
G. Lahoud, V. Timoshchuk, A. Lebedev, M. de Vega, M. Salas, K. Arar, Y.-M. Hou, and H. Gamper
Enzymatic synthesis of structure-free DNA with pseudo-complementary properties
Nucleic Acids Res., June 1, 2008; 36(10): 3409 - 3419.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.