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Nucleic Acids Research Advance Access originally published online on January 23, 2007
Nucleic Acids Research 2007 35(3):930-938; doi:10.1093/nar/gkl1145
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Nucleic Acids Research, 2007, Vol. 35, No. 3 930-938
© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Nucleic Acid Enzymes

GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase

Sandra Wydau, Maria-Laura Ferri-Fioni, Sylvain Blanquet and Pierre Plateau*

Laboratoire de Biochimie, UMR CNRS 7654, Département de Biologie, Ecole Polytechnique, 91128 Palaiseau Cedex, France

*To whom correspondence should be addressed: Tel: +33 1 69 33 41 81; Fax: +33 1 69 33 30 13; Email: plateau{at}bioc.polytechnique.fr

Received July 26, 2006. Revised October 19, 2006. Accepted December 15, 2006.

GEK1, an Arabidopsis thaliana gene product, was recently identified through its involvement in ethanol tolerance. Later, this protein was shown to display 26% strict identity with archaeal D-Tyr-tRNATyr deacylases. To determine whether it actually possessed deacylase activity, the product of the GEK1 open reading frame was expressed in Escherichia coli from a multi-copy plasmid. Purified GEK1 protein contains two zinc ions and proves to be a broad-specific, markedly active D-aminoacyl-tRNA deacylase in vitro. Moreover, GEK1 expression is capable of functionally compensating in E. coli for the absence of endogeneous D-Tyr- tRNATyr deacylase. Possible connections between exposure of plants to ethanol/acetaldehyde and misaminoacylation of tRNA by D-amino acids are considered.


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