Nucleic Acids Research Advance Access originally published online on January 23, 2007
Nucleic Acids Research 2007 35(4):e24; doi:10.1093/nar/gkl1129
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Nucleic Acids Research, 2007, Vol. 35, No. 4 e24
© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method
1Department of Chemistry and Biotechnology, Graduate School of Engineering and 2Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
*To whom correspondence should be addressed: Tel: +81 3 5841 8752; Fax: +81 3 3816 0106; E-mail: ts{at}chembio.t.u-tokyo.ac.jp
Received November 2, 2006. Revised December 11, 2006. Accepted December 11, 2006.
Recent genome-wide transcriptome analysis has identified diverse classes of non-coding RNAs (ncRNAs), some of which have been demonstrated to be functional, regulatory RNAs involved in various biological processes. Maturation of RNA molecules through various post-transcriptional processing events, including splicing, modification, editing and trimming of both ends, is required for correct folding and proper function of RNA molecules. To characterize post-transcriptional modifications and terminal chemical structures of fully processed native RNAs, it is necessary to isolate individual RNA species from a limited quantity and complex mixture of cellular RNAs. However, there have been no general and convenient strategies for isolation of individual RNAs. We describe here the first example of automated parallel isolation of individual ncRNAs using a novel method named ‘reciprocal circulating chromatography (RCC)’. RCC employs multiple tip-columns packed with solid-phase DNA probes to isolate multiple RNA species from a common sample of total RNAs. A pilot RCC instrument successfully isolated various ncRNAs from E. coli, yeast and mouse.
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