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Nucleic Acids Research Advance Access originally published online on February 20, 2007
Nucleic Acids Research 2007 35(5):1673-1686; doi:10.1093/nar/gkl1112
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Nucleic Acids Research, 2007, Vol. 35, No. 5 1673-1686
Published by Oxford University Press 2007.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Ubiquitin-interaction motifs of RAP80 are critical in its regulation of estrogen receptor {alpha}

Jun Yan1, Yong-Sik Kim1, Xiao-Ping Yang1, Michael Albers2, Manfred Koegl3 and Anton M. Jetten1,*

1Cell Biology Section, Division of Intramural Research, National Institute of Enironmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA, 2Phenex Pharmaceuticals AG, D-67056 Ludwigshafen, Germany and 3RZPD German Resource Center for Genome Research, D-69120 Heidelberg, Germany

*To whom correspondence should be addressed. Tel: 919-541-2768; Fax: 919-541-4133; E-mail: jetten{at}niehs.nih.gov

Received June 8, 2006. Revised November 10, 2006. Accepted December 6, 2006.

In this study, we demonstrate that receptor-associated protein 80 (RAP80) interacts with estrogen receptor alpha (ER{alpha}) in an agonist-dependent manner. The interaction is specific for ER{alpha} as ERß and several other nuclear receptors tested did not interact with RAP80. Interaction between RAP80 and ER{alpha} was supported by mammalian two-hybrid, GST pull-down, and co-immunoprecipitation analyses. The hinge/ligand-binding domain of ER{alpha} is sufficient for interaction with RAP80. RAP80 overexpression reduces ER{alpha} polyubiquitination, increases the level of ER{alpha} protein, and enhances ER{alpha}-mediated transactivation. Knockdown of endogenous RAP80 expression by small-interfering RNA (siRNA) reduced ER{alpha} protein level and the E2-dependent induction of pS2. In this study, we also demonstrate that RAP80 contains two functional ubiquitin-interaction motifs (UIMs) that are able to bind ubiquitin and to direct monoubiquitination of RAP80. Deletion of these UIMs does not affect the ability of RAP80 to interact with ER{alpha}, but eliminates the effects of RAP80 on ER{alpha} polyubiquitination, the level of ER{alpha} protein, and ER{alpha}-mediated transcription. These data indicate that the UIMs in RAP80 are critical for the function of RAP80. Our study identifies ER{alpha} as a new RAP80-interacting protein and suggests that RAP80 may be an important modulator of ER{alpha} activity.


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