Skip Navigation


Nucleic Acids Research Advance Access originally published online on February 8, 2007
Nucleic Acids Research 2007 35(6):e42; doi:10.1093/nar/gkm042
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (520K) Freely available
Right arrow Screen PDF (195K) Freely available
Right arrowOA All Versions of this Article:
35/6/e42    most recent
gkm042v3
gkm042v2
gkm042v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Macaskill, A.
Right arrow Articles by Graham, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Macaskill, A.
Right arrow Articles by Graham, D.
Related Collections
Right arrow Miscellaneous/other
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2007, Vol. 35, No. 6 e42
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides

A. Macaskill1, A. A. Chernonosov2,3, V. V. Koval2, E. A. Lukyanets4, O. S. Fedorova2, W. E. Smith1, K. Faulds1,* and D. Graham1,{dagger}

1Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK G1 1XL, 2Institute of Chemical Biology and Fundamental Medicine, Lavrentyev Avenue 8, Novosibirsk 630090, Russia, 3Institute of Human Ecology, Sovetskii Avenue 18, Kemerovo 650099, Russia and 4Organic Intermediates & Dyes Institute, B. Sadovaya, 1/4, Moscow 103787, Russia

*To whom correspondence should be addressed. Tel: 0141 548 4701; Fax: 0141 552 0876; Email: Karen.Faulds{at}strath.ac.uk

{dagger}Correspondence may also be addressed to D. Graham. Tel: 0141 548 4701; Fax: 0141 552 0876; Email: Duncan.Graham{at}strath.ac.uk

Received November 28, 2006. Revised January 9, 2007. Accepted January 9, 2007.

The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10–11 mol. dm–3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.