Nucleic Acids Research Advance Access originally published online on March 13, 2007
Nucleic Acids Research 2007 35(7):e52; doi:10.1093/nar/gkl1118
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Nucleic Acids Research, 2007, Vol. 35, No. 7 e52
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Methods Online |
A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs
1Novartis Institutes for BioMedical Research, Genome and Proteome Sciences, CH-4002 Basel, Switzerland, 2Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH-4002 Basel, Switzerland and 3Novartis Pharma AG, Biomarker Development, CH-4002 Basel, Switzerland
*To whom correspondence should be addressed. Tel: +49-61 3246142; Fax: +41-61-3242217; Email: jan.weiler{at}novartis.com Correspondence may also be addressed to Witold Filipowicz. Tel: +41-61 6974128; Fax: +41-61-6973976; E-mail: witold.filipowicz{at}fmi.ch
Received August 8, 2006. Revised October 30, 2006. Accepted February 27, 2007.
Microarrays to examine the global expression levels of microRNAs (miRNAs) in a systematic in-parallel manner have become important tools to help unravel the functions of miRNAs and to understand their roles in RNA-based regulation and their implications in human diseases. We have established a novel miRNA-specific microarray platform that enables the simultaneous expression analysis of both known and predicted miRNAs obtained from human or mouse origin. Chemically modified 2'-O-(2-methoxyethyl)-(MOE) oligoribonucleotide probes were arrayed onto Evanescent Resonance (ER) microchips by robotic spotting. Supplementing the complementary probes against miRNAs with carefully designed mismatch controls allowed for accurate sequence-specific determination of miRNA expression profiles obtained from a panel of mouse tissues. This revealed new expression signatures of known miRNAs as well as of novel miRNAs previously predicted using bioinformatic methods. Systematic confirmation of the array data with northern blotting and, in particular, real-time PCR suggests that the described microarray platform is a powerful tool to analyze miRNA expression patterns with rapid throughput and high fidelity.
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.
Present address: Fabrice A. Kolb, Protease Platform, Novartis Institutes for Biomedical Research, CH-4002 Basel, Switzerland Jonathan Hall, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH) Zurich, Switzerland
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