Skip Navigation


Nucleic Acids Research Advance Access originally published online on March 29, 2007
Nucleic Acids Research 2007 35(8):2473-2482; doi:10.1093/nar/gkm096
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1638K) Freely available
Right arrow Screen PDF (403K) Freely available
Right arrowOA All Versions of this Article:
35/8/2473    most recent
gkm096v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Petz, M.
Right arrow Articles by Mikulits, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petz, M.
Right arrow Articles by Mikulits, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2007, Vol. 35, No. 8 2473-2482
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

The leader region of Laminin B1 mRNA confers cap-independent translation

Michaela Petz1, Daniela Kozina2, Heidemarie Huber1, Tanja Siwiec1, Joachim Seipelt3, Wolfgang Sommergruber2 and Wolfgang Mikulits1,*

1Department of Medicine I, Division: Institute of Cancer Research, Medical University of Vienna, Borschke-Gasse 8a, A-1090 Vienna, 2Boehringer Ingelheim Austria, Dr Boehringer Gasse 5-10, A-1120 Vienna and 3Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Medical Biochemistry, Medical University of Vienna, Dr Bohr-Gasse 9, A-1030 Vienna, Austria

*To whom correspondence should be addressed. Tel: +43 1 4277 65250; Fax: +43 1 4277 65239; Email: wolfgang.mikulits{at}meduniwien.ac.at

Received November 23, 2006. Revised February 2, 2007. Accepted February 3, 2007.

Translation initiation of eukaryotic mRNAs generally occurs by cap-dependent ribosome scanning. However, certain mRNAs contain internal ribosome entry sites (IRES) allowing cap-independent translation. Several of these IRES-competent transcripts and their corresponding proteins are involved in tumourigenesis. This study focused on IRES-driven translation control during the epithelial to mesenchymal transition (EMT) of hepatocytes that reflects crucial aspects of carcinoma progression. Expression profiling of EMT revealed Laminin B1 (LamB1) to be translationally upregulated. The 5'-untranslated region (UTR) of LamB1 was potent to direct IRES-dependent mRNA utilization of a bicistronic reporter construct. Stringent assays for cryptic promoter and splice sites showed no aberrantly expressed transcripts, suggesting that the reporter activity provided by the leader region of LamB1 mRNA exclusively depends on IRES. In accordance, LamB1 expression increased upon negative interference with cap-dependent translation by expression of human rhinovirus 2A protease or heat shock of cells. Finally, the enhanced expression of LamB1 during EMT correlated with an elevated IRES activity. Together, these data provide first evidence that the 5'-UTR of LamB1 contains a bona fide IRES that directs translational upregulation of LamB1 during stress conditions and neoplastic progression of hepatocytes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
H. A. Saffran and J. R. Smiley
The XIAP IRES activates 3' cistron expression by inducing production of monocistronic mRNA in the {beta}gal/CAT bicistronic reporter system
RNA, November 1, 2009; 15(11): 1980 - 1985.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.