Nucleic Acids Research Advance Access originally published online on October 26, 2006
Nucleic Acids Research 2007 35(Database issue):D805-D809; doi:10.1093/nar/gkl767
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Nucleic Acids Research, 2007, Vol. 35, Database issue D805-D809
Published by Oxford University Press 2006
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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qPrimerDepot: a primer database for quantitative real time PCR
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute Bethesda, MD 20892-4605 1 Laboratory of Immunology, National Institute on Aging Baltimore, MD 21224, USA
*To whom correspondence should be addressed at National Institutes of Health, Advanced Technology Center, Room 134C, 8717 Grovemont Circle, Bethesda, MD 20892-4605, USA. Tel: +1 301 496 1055; Fax: +1 301 435 7558; Email: gardnerk{at}mail.nih.gov
Received June 3, 2006. Revised September 25, 2006. Accepted September 29, 2006.
Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.
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