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Nucleic Acids Research Advance Access originally published online on August 30, 2008
Nucleic Acids Research 2008 36(17):5562-5570; doi:10.1093/nar/gkn505
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Nucleic Acids Research, 2008, Vol. 36, No. 17 5562-5570
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Chemistry and Synthetic Biology

Kinetics of charge transfer in DNA containing a mismatch

Yasuko Osakada, Kiyohiko Kawai*, Mamoru Fujitsuka and Tetsuro Majima

The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki Osaka 567-0047, Japan

*To whom correspondence should be addressed. Tel: +81 6 6879 8496; Fax: +81 6 6879 8499; Email: kiyohiko{at}sanken.osaka-u.ac.jp

Correspondence may also be addressed to Tetsuro Majima. Tel: +81 6 6879 8495; Fax: +81 6 6879 8499; Email: majima{at}sanken.osaka-u.ac.jp

Received May 16, 2008. Revised July 22, 2008. Accepted July 22, 2008.

Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch.


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