Nucleic Acids Research Advance Access originally published online on November 12, 2008
Nucleic Acids Research 2008 36(22):7240-7251; doi:10.1093/nar/gkn898
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Nucleic Acids Research, 2008, Vol. 36, No. 22 7240-7251
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
RNA |
Small non-coding RNAs in Streptomyces coelicolor
1Department of Biology and Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1, and 2Computer Science Department, Wellesley College, Wellesley, MA 02481, USA
*To whom correspondence should be addressed. Tel: +1 905 525 9140 (ext. 24225); Fax: +1 905 522 6066; Email: melliot{at}mcmaster.ca
Received August 25, 2008. Revised October 23, 2008. Accepted October 24, 2008.
In bacteria, small RNAs (sRNAs) make important regulatory contributions to an ever increasing number of cellular processes. To expand the repertoire of known sRNAs, we sought to identify novel sRNAs in the differentiating, multicellular bacterium Streptomyces coelicolor. We describe a combined bioinformatic and experimental approach that enabled the identification and characterization of nine novel sRNAs in S. coelicolor, including a cis-encoded antisense sRNA. We examined sRNA expression throughout the S. coelicolor developmental cycle, which progresses from vegetative mycelium formation, to aerial mycelium formation and finally sporulation. We further determined the effects of growth medium composition (rich versus minimal medium) on sRNA gene expression, and compared wild-type sRNA expression profiles with those of four developmental mutants. All but two of the sRNAs exhibited some degree of medium dependence, with three sRNAs being expressed exclusively during growth on one medium type. Unlike most sRNAs characterized thus far, several sRNA genes in S. coelicolor were expressed constitutively (apart from during late sporulation), suggesting a possible housekeeping role for these transcripts. Others were expressed at specific developmental stages, and their expression profiles were altered in response to developmental mutations. Expression of one sRNA in particular was dependent upon the sporulation-specific sigma factor 
WhiG.
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. J. Haiser, M. R. Yousef, and M. A. Elliot Cell Wall Hydrolases Affect Germination, Vegetative Growth, and Sporulation in Streptomyces coelicolor J. Bacteriol., November 1, 2009; 191(21): 6501 - 6512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tezuka, H. Hara, Y. Ohnishi, and S. Horinouchi Identification and Gene Disruption of Small Noncoding RNAs in Streptomyces griseus J. Bacteriol., August 1, 2009; 191(15): 4896 - 4904. [Abstract] [Full Text] [PDF]
|
|