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Nucleic Acids Research Advance Access originally published online on February 11, 2008
Nucleic Acids Research 2008 36(6):1861-1870; doi:10.1093/nar/gkn031
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Nucleic Acids Research, 2008, Vol. 36, No. 6 1861-1870
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Identification of CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis

Antoine Graindorge1, Olivier Le Tonquèze1, Raphaël Thuret2, Nicolas Pollet2, H. Beverley Osborne1 and Yann Audic1,*

1CNRS, UMR 6061 Génétique et Développement, Université de Rennes 1, IFR 140 GFAS, 2 avenue du Pr Léon Bernard, CS 34317, 35043 Rennes Cedex and 2CNRS UMR 8080, Université Paris Sud, Orsay, France

*To whom correspondence should be addressed. Tel: 33 2 2323 4475; Fax: 33 2 2323 4478; Email: yann.audic{at}univ-rennes1.fr

Received October 5, 2007. Revised January 16, 2008. Accepted January 19, 2008.

The early development of many animals relies on the posttranscriptional regulations of maternally stored mRNAs. In particular, the translation of maternal mRNAs is tightly controlled during oocyte maturation and early mitotic cycles in Xenopus. The Embryonic Deadenylation ElemeNt (EDEN) and its associated protein EDEN-BP are known to trigger deadenylation and translational silencing to several mRNAs bearing an EDEN. This Xenopus RNA-binding protein is an ortholog of the human protein CUG-BP1/CELF1. Five mRNAs, encoding cell cycle regulators and a protein involved in the notch pathway, have been identified as being deadenylated by EDEN/EDEN-BP. To identify new EDEN-BP targets, we immunoprecipitated EDEN-BP/mRNA complexes from Xenopus tropicalis egg extracts. We identified 153 mRNAs as new binding targets for EDEN-BP using microarrays. Sequence analyses of the 3' untranslated regions of the newly identified EDEN-BP targets reveal an enrichment in putative EDEN sequences. EDEN-BP binding to a subset of the targets was confirmed both in vitro and in vivo. Among the newly identified targets, Cdk1, a key player of oocyte maturation and cell cycle progression, is specifically targeted by its 3' UTR for an EDEN-BP-dependent deadenylation after fertilization.

Present address: Antoine Graindorge, Centre de Regulació Genómica (CRG-UPF), 08003 Barcelona, Spain


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