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Nucleic Acids Research Advance Access originally published online on October 4, 2007
Nucleic Acids Research 2008 36(Database issue):D1044-D1047; doi:10.1093/nar/gkm780
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Nucleic Acids Research, 2008, Vol. 36, Database issue D1044-D1047
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Articles

Shanghai RAPESEED Database: a resource for functional genomics studies of seed development and fatty acid metabolism of Brassica

Guo-Zhang Wu, Qiu-Ming Shi, Ya Niu, Mei-Qing Xing and Hong-Wei Xue*

National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science (SIBS), Chinese Academy of Sciences, China

*To whom correspondence should be addressed. Tel: +86 21 54924059; Fax: +86 21 54924060; Email: hwxue{at}sibs.ac.cn

Received August 4, 2007. Revised September 14, 2007. Accepted September 17, 2007.

The Shanghai RAPESEED Database (RAPESEED, http://rapeseed.plantsignal.cn/) was created to provide the solid platform for functional genomics studies of oilseed crops with the emphasis on seed development and fatty acid metabolism. The RAPESEED includes the resource of 8462 unique ESTs, of which 3526 clones are with full length cDNA; the expression profiles of 8095 genes and the Serial Analysis of Gene Expression (SAGE, 23 895 unique tags) and tag-to-gene data during seed development. In addition, a total of ~14 700 M3 mutant populations were generated by ethylmethanesulfonate (EMS) mutagenesis and related seed quality information was determined using the Foss NIR System. Further, the TILLING (Targeting Induced Local Lesions IN Genomes) platform was established based on the generated EMS mutant population. The relevant information was collected in RAPESEED database, which can be searched through keywords, nucleotide or protein sequences, or seed quality parameters, and downloaded.


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