Skip Navigation


Nucleic Acids Research Advance Access originally published online on January 16, 2009
Nucleic Acids Research 2009 37(4):1323-1334; doi:10.1093/nar/gkn1041
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (6100K) Freely available
Right arrow Screen PDF (860K) Freely available
Right arrow Supplementary Data
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
37/4/1323    most recent
gkn1041v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sleumer, M. C.
Right arrow Articles by Jones, S. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sleumer, M. C.
Right arrow Articles by Jones, S. J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2009, Vol. 37, No. 4 1323-1334
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Genomics

Caenorhabditis elegans cisRED: a catalogue of conserved genomic elements

Monica C. Sleumer, Mikhail Bilenky, An He, Gordon Robertson, Nina Thiessen and Steven J. M. Jones*

Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, Canada, V5Z 4S6

*To whom correspondence should be addressed. Tel: +1 604 877 6083; Fax: +1 604 876 3561; Email: sjones{at}bcgsc.ca

Received October 14, 2008. Revised December 11, 2008. Accepted December 12, 2008.

The availability of completely sequenced genomes from eight species of nematodes has provided an opportunity to identify novel cis-regulatory elements in the promoter regions of Caenorhabditis elegans transcripts using comparative genomics. We determined orthologues for C. elegans transcripts in C. briggsae, C. remanei, C. brenneri, C. japonica, Pristionchus pacificus, Brugia malayi and Trichinella spiralis using the WABA alignment algorithm. We pooled the upstream region of each transcript in C. elegans with the upstream regions of its orthologues and identified conserved DNA sequence elements by de novo motif discovery. In total, we discovered 158 017 novel conserved motifs upstream of 3847 C. elegans transcripts for which three or more orthologues were available, and identified 82% of 44 experimentally proven regulatory elements from ORegAnno. We annotated 26% of the motifs as similar to known binding sequences of transcription factors from ORegAnno, TRANSFAC and JASPAR. This is the first catalogue of annotated conserved upstream elements for nematodes and can be used to find putative regulatory elements, improve gene models, discover novel RNA genes, and understand the evolution of transcription factors and their binding sites in phylum Nematoda. The annotated motifs provide novel binding site candidates for both characterized transcription factors and orthologues of characterized mammalian transcription factors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.