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Nucleic Acids Research Advance Access originally published online on February 17, 2009
Nucleic Acids Research 2009 37(7):2065-2069; doi:10.1093/nar/gkp056
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Nucleic Acids Research, 2009, Vol. 37, No. 7 2065-2069
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RDML: structured language and reporting guidelines for real-time quantitative PCR data

Steve Lefever1, Jan Hellemans1,*, Filip Pattyn1, Daniel R. Przybylski2, Chris Taylor3, René Geurts4, Andreas Untergasser4, Jo Vandesompele1 and on behalf of the RDML consortium

1Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium, 2Bio-Rad Laboratories, Inc., Hercules, CA, USA, 3European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SD, UK and 4Laboratory of Molecular Biology, Department of Plant Science, Wageningen University, The Netherlands

*To whom correspondence should be addressed. Tel: +32 9 3320158; Fax: +32 9 3326549; Email: info{at}rdml.org or jan.hellemans{at}ugent.be

Received October 7, 2008. Revised December 31, 2008. Accepted January 20, 2009.

The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exchange of qPCR data and related information between qPCR instruments and third party data analysis software, between colleagues and collaborators and between experimenters and journals or public repositories. We here also propose data related guidelines as a subset of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) to guarantee inclusion of key data information when reporting experimental results.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


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J. Vermeulen, F. Pattyn, K. De Preter, L. Vercruysse, S. Derveaux, P. Mestdagh, S. Lefever, J. Hellemans, F. Speleman, and J. Vandesompele
External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
Nucleic Acids Res., September 4, 2009; (2009) gkp721v1.
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