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Nucleic Acids Research, 1977, Vol. 4, No. 1 207-221
© 1977

Articles

The iminoprotan NMR spectrum of yeast tRNAPhe predicted from crystal coordinates

H.A.M. Geerdes and C.W. Hilbers

Laboratory of Biophysical Chemistry, University of Nijmegen Toernooiveld, Nijmegen, The Netherlands

Received November 4, 1976. The ring current effects on the base paired iminoprotons in yeast tRNAPhe have been calculated from crystal coordinates. The results in conjunction with independently determined intrinsic positions of the iminoprotons in various base pairs enable us to predict the low field NMR spectrum of yeast tRNAPhe. It turns out that the calculated NMR spectra are very sensitive to slight changes in structure. Moreover the crystal and solution structure are identical as far as the present methods go.


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