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Nucleic Acids Research, 1977, Vol. 4, No. 1 241-246
© 1977

Articles

Removal of RNase activity from DNase by affinity chromatography on agarose-coupled aminophenylphosphoryl-uridlne-2'(3')-phosphate

Ian H. Maxwell, Francoise Maxwell and William E. Hahn

Dempartment of Anatomy, University of Colorado School of Medicine Denver, CO 80262, USA

Received November 11, 1976. Severe degradation of high molecular weight RNA was shown to occur during incubation with commercially purified DNase. Most of the RNase activity could be removed by passage of the DNase through a column of agarose-coupled amino-phenylphosphoryl-uridine-2'(3')-phosphate. Incubation with the treated DNase caused only minimal alteration of the sedimentation pattern of high molecular weight nuclear RNA, determined under partially denaturing conditions. No impairment of DNase activity was detected.


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[Abstract] [PDF]


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