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Nucleic Acids Research, 1977, Vol. 4, No. 10 3483-3496
© 1977

Articles

Studies on the biological role of DNA methylation: III. Role in excision of one-genome long single-stranded øX174 DNA

Joseph Friedman, Adam Friedmann* and Aharon Razin+

Department of Cellular Biochemistry, The Hebrew University-Hadassah Medical School Jerusalem, Israel 91000 *Department of Genetics, The Hebrew University Jerusalem, Israel +On Sabbatical leave at the Department of Biology, City of Hope Medical Center, 1500 E. Duarte Road., Duarte, CA 91010, USA.

Received July 5, 1977. Accumulation of replicative intermediates of the bacteriophage øX174 was observed in E.coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron microscopy revealed that these molecules are composed of double-stranded DNA rings with multiple-genome length single-stranded "tails". These results suggest that the single 5-methylcytosine residue present in the phage DNA serves as a recognition site for the gene A protein mediating the excision of one-genome long phage DNA. This excision process is obligatory for the final maturation of the phage.


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