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Nucleic Acids Research, 1977, Vol. 4, No. 10 3511-3517
© 1977

Articles

A rapid method for preparation of calf spleen exonuclease*

S. Silverman and D. Soll

Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06520, USA

Received July 13, 1977.

Chromatography on Concanaval in A Sepharose is a simple procedure for partial purification of spleen phosphodiesterase. This procedure removes much, but not all, of the ribonuclease activity found as contaminant in most spleen phosphodiesterase preparations.

*This work was supported by grants from the National Institutes of Health (GM 22854 and HD 09167).


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