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Nucleic Acids Research, 1977, Vol. 4, No. 10 3573-3580
© 1977

Articles

DNA double-strand breaks and alkali-labile bonds produced by bleomycin

Lawrence F. Povirk*, Werner Wübker{dagger}, Wolfgang Köhnlein{dagger} and Franklin Hutchinson*,{dagger}

*Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06520, USA {dagger}Institut für Strahlenbiologie, Universität Münster 44 Münster, GFR

{dagger}author to whom correspondence should be addressed

Received July 28, 1977. Both in linear T2 DNA, analyzed by velocity sedimentation, and in supercoiled Col E1 DNA, analyzed by gel electrophoresis, the number of double-strand breaks produced by bleomycin was directly propotional to the number of single-strand breaks and was far greater than the number expected from random coincidence of single-strand breaks, suggesting that the bleomycin-induced double-strand breaks occur as an independent event. In Col E1 DNA, at least twice as many single-strand breaks were found under alkaline assay conditions as were found under neutral conditions, showing the production of alkaline-labile bonds by bleomycin.


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