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Nucleic Acids Research, 1977, Vol. 4, No. 11 3679-3686
© 1977

Articles

Interactions of norharman and harman with DNA

Kenshi Hayashi, Minako Nagao and Takashi Sugimura

Biochemistry Division, National Cancer Center Research Institute Tsukiji 5-1-1, Chuo-ku, Tokyo, Japan

Received August 15, 1977. The interactions of norharman (9H-pyrido[3,4-b]indole) and harman (1-methyl-9H-pyrido[3,4-b]indolel with DNA were studied. DNA caused remarkable fluorescence quenching and change in the absorption spectra of the dyes. Scatchard plots obtained by optical titration gave Kd values of 2.2 x 10–5M and 7.7 x 10–6M, and apparent numbers of binding sites of 0.13/base and 0.12/base for norharman and harman, respectively. Agarose gel electro-phoresis of circular DNA, closed in the presence or absence of norharman revealed that the dye intercalates DNA, thereby causing 17 ± 3° unwinding of the double helix.


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