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Nucleic Acids Research, 1977, Vol. 4, No. 11 3727-3742
© 1977

Articles

Demonstration of a tertiary interaction in solution between the extra arm and the D-stem in two different transfer RNA's by NMR

P. J. M. Salemink, T. Yamane* and C. W. Hilbers

Department of Biophysical Chemistry, University of Nijmegen Nijmegen, Netherlands

Received July 20, 1977.

According to the X-ray structure of yeast tRNAPhe at 2.5 å resolution, a hydrogen bond is formed between m7G46 and G22 By removal of this m7G46-residue we demonstrate that this interaction is present in solution as well. Comparison of the 1H 360 MHz NMR spectra of intact yeast tRNAphe and its m7G-excised derivative locates the position of this tertiary H-bond at 12.5 ppm downfield from DSS. Additional evidence for the presence of this interaction in solution comes from a comparison of 1H NMR spectra of E. coli Formula and E. coli Formula, which differ only in a single position in the extra arm. in Formula residue 47 is a m7G-residue. whereas in Formula it is A, resulting in the absence of the m7G47-G23-C13 triple interaction, characteristic of Formula. The resonance position of this tertiary interaction in Formula is located around –13.6 ppm, a chemical shift difference of 1.1 ppm with respect to the position observed for tRNAPhe. The origin of this chemical shift difference is discussed in relation to the structure of their respective augmented D-helices.

*Present adress: Bell Laboratories, Murray Hill, N.J., USA.


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R. Oliva, L. Cavallo, and A. Tramontano
Accurate energies of hydrogen bonded nucleic acid base pairs and triplets in tRNA tertiary interactions
Nucleic Acids Res., February 6, 2006; 34(3): 865 - 879.
[Abstract] [Full Text] [PDF]


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