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Nucleic Acids Research, 1977, Vol. 4, No. 11 3787-3802
© 1977

Articles

Determination of the sequence homology between the four BNA species of cucumber mosaic virus by hybridization analysis with complementary DNA

Allan R. Gould and Robert H. Symors*

Department of Biochemistry, University of Adelaide Adelaide, South Australia, 5001

*author to whom correspondence should be addressed

Received July 27, 1977. The method of Taylor et al., (11) has been used to transcribe complementary DNA probes from the four major RNA species of cucumber mosaic virus (RNAs 1 – 4 in order of decreasing molecular weight). Analysis of the kinetics of hybridization of these probes in homologous and heterologous complementary DNA-RNA hybridization reactions has shown that the sequence of the smallest RNA (RNA 4), which contains the coat protein gene, is present within RNA 3. RNAs 1 and 2 are unique RNA molecules while each has a region of approximately 300 nucleotides in common with RNA 4.


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