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Nucleic Acids Research, 1977, Vol. 4, No. 11 3821-3828
© 1977

Articles

Urea-induced structural changes in chromatin obtained by sedimentation

Rodney E. Harrington*

The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences and the Biology Division, Oak Ridge National Laboratory Oak Ridge, TN 37830, USA

Received August 8, 1977.

Sedimentation coefficients have been determined for fractionated preparations of whole and stripped (depleted of very lysine-rich histones and non-histone proteins) chicken erythrocyte chromatin fragments in 0–10 M urea. Significant differences in urea effects are observed between these preparations; differences which can be interpreted structurally by use of Kirkwood's dynamical theory of the translational frictional coefficient. This type of analysis implies that urea-induced chain-swelling in stripped chromatin is due largely to the urea effect upon the constituent v-bodies, whereas the much larger swelling observed in whole chromatin appears to involve also the effect of urea upon the region between adjacent v-bodies.

*During this work the author was on leave (1976–77) from the Department of Chemistry, University of Nevada, Reno, Nevada 89507. Please address correspondence and reprint requests to him there. Oak Ridge National Laboratory is operated by Union Carbide Corporation for the Energy Research and Development Administration.


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