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Nucleic Acids Research, 1977, Vol. 4, No. 11 4053-4062
© 1977

Articles

Specific fragmentation of T7 phage DNA at low-melting sites

V.M pavlov, Yu.L.L. Lyubchenko, A.S. Borovik and Yu.S. Lazurkin

Department of Biology, I.V.Kurchatov Institute of Atomic Energy Moscow, USSR

Received October 18, 1977. A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.


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L. Shokri, M. J. McCauley, I. Rouzina, and M. C. Williams
DNA Overstretching in the Presence of Glyoxal: Structural Evidence of Force-Induced DNA Melting
Biophys. J., August 1, 2008; 95(3): 1248 - 1255.
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