Specificity of Hpa II and Hae II DNA methylases

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Nucleic Acids Research, 1977, Vol. 4, No. 12 4211-4222
© 1977

Articles

Specificity of Hpa II and Hae II DNA methylases

Michael B. Mann and Hamilton O. Smith

Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Received October 31, 1977. The methylases M·HaeIII and M·HpaII recognizethe tetranucleotide sequences Formula and Formula respectively, in DNA, and transfer a methyl group from 5-adenosylmethionine to the5-position of cytosine on each strand as indicated by the asterisks.Restriction endonuclease R·HaeIII does not cleave themethylated sequence Formula but can cleave Formula in which methylation is introduced on the unn external cytosine positions. Similarly,R·HpaII does not cleave Formula but can cleave Formula .

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