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Nucleic Acids Research, 1977, Vol. 4, No. 12 4305-4312
© 1977

Articles

A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts

Mary Sue Colemán

Department of Biochemistry, University of Kentucky Lexington, KY 40506, USA

Received September 16, 1977. Terminal deoxynucleotidyl transferase (TdT) is a non-template directed DNA polymerase normally found in vertebrate thymus and bone marrow. Quantitative assay of TdT activity is being widely used as a tool in the differential diagnosis of acute leukemias in man. Clinical specimens of blood and bone marrow often contain 107 or fewer cells and require a specific and rapid assay for transferase which can be carried out in crude cell extracts. Commonly used assay methods do not meet these requirements, but can be easily modified to do so.


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