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Nucleic Acids Research, 1977, Vol. 4, No. 12 4411-4424
© 1977

Articles

Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria

O.I. Kisselev, V.S. Gaitskhoki and S.A. Neifakh

Laboratory of Biochemical Genetics, Institute of Experimental Medicine 197022 Leningrad, USSR

Received November 3, 1977. Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal. supernatant of rat liver mitochondria. Both mRRA's contain rather short poly(A) tracts (40–70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated. mitochondrial lysate programmed. the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltona which was soluble in the neutral chloroform-methanol mixture.


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