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Nucleic Acids Research, 1977, Vol. 4, No. 3 625-639
© 1977

Articles

Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina

Herman Slegers and Masatoshi Kondo*

Department of Cell Biology, University of Antwerp B-2610 Wilrijk, Belgium

*The author to whom all correspondence should be addressed.

Received December 20, 1976. Poly(A)-containing ribonucleoprotein [poly(A)+•RNP) particles in the post-mitochondrial supernatant of cryptobiotic embryos of Artemia salina were characterized by hybridization to [3H]poly(U). By sucrose isopycnic centrifugation, approximately 2/3 of poly(A)+•RNPs was found to band at 1.27–1.30 (g/cm3) and the rest 1/3 at 1.20–1.23 (g/cm3) and below 1.20 (g/cm3). The 1.27–1.30 RNP8 could be separated into two density classes, 1.27–1.26 and 1.30 (g/cm3) respectively. The latter RNP class was apparently complexed with ribosomal components because they were completely converted to the former RNP class [free RNPs) by 25 mM EDTA treatment. Further, the 1.30 (g/cm3) RNPs were res-solved into several RNP species having sedimentation coefficients above 50 S, which were transformed mostly to 20–30 S RNPs in the presence of 25 mM EDTA. The free 20–30 S RNPs contained 8–14 S poly(A)+RNAs, having the highest template activity in a wheat embryo cell-free system, whereas the 1.20–1.23 poly(A)±RNPs consisted of 10 S and 16 S RNPs, both of which contained 4 S poly(A)-containing sequences without any template activity.


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