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Nucleic Acids Research, 1977, Vol. 4, No. 3 747-759
© 1977

Articles

Synthesis of oltgonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Es cherichia coli: preparation of m62A-C-C-U-C-C and A-C-C-U-C-m42C via phosphotriester intermediates1

J.H. van Boom, P.M.J. Burgers, R. Crea, G. van der Marel and G. Wille

Department of Organic Chemistry, Gorlaeus Laboratories, State University of Leiden PO Box 75, Leiden, The Netherlands

Received February 4, 1977. The synthesis of two fully-protected hexanucleotides [11a and 11b] via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotide linkages, i.e. one 2,2,2-tribromo-ethyl at the 57'-terminus and four 2-chlorophenyl groups for the remaining linkages, is reported. The hexanucleotides 11a and 11b, assembled via a block-wise two-step phosphotriester method, can be deblocked conveniently to give the two hexamers 12a and 12b containing only 3'->5' internucleotide linkages.


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