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Nucleic Acids Research, 1977, Vol. 4, No. 4 1001-1014
© 1977

Articles

Nucleotide sequence of the restriction fragment Hind F - Eco RI2 of SV40 DNA

R. Contreras, G. Volckaert, F. Thys, A. Van de Voorde and W. Fiers

Laboratory of Molecular Biology and Laboratory of Physiological Chemistry, University of Ghent Ledeganckitraat, 35.9000 Gent, Belgium

Received February 22, 1977. The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.


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