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Nucleic Acids Research, 1977, Vol. 4, No. 5 1267-1272
© 1977

Articles

Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA*

Lawrence I. Grossman1, Richard C. Parker2, Robert M. Watson3, Sarah E.W. Chandler1 and Marlyn Teplitz3

1Department of Biochemistry, Wayne State University School of Medicine Detroit, Ml 48201 2Division of Biology, California Institute of Technology Pasadena, CA 91125. USA 3Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena. CA 91125. USA

Received February 10, 1977.

A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.

*Dedicated to the memory of Professor Jerome Vinograd.


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