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Nucleic Acids Research, 1977, Vol. 4, No. 5 1419-1428
© 1977

Articles

Absence of ethidium bromide induced nicking and degradation of mitochondrial DNA in mouse L-cells

Charles Allen Smith

Department of Biological Sciences, Stanford University Stanford, CA 94305, USA

Received January 3, 1977. The in vivo effects of ethidium bromide on the integrity of mitochondrial DNA have been studied in a mouse L-cell system in which this DNA may be nearly exclusively radiolabelled. This allows the detection of mitochondrial DNA in the presence of contaminating nuclear DNA and eliminates the need for extensive purification of mitochondria or the use of deoxyribonuclease. The mitochondrial DNA in treated cells rapidly attains a high negative superhelix density and is not substantially nicked or degraded over the course of several days.


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