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Nucleic Acids Research, 1977, Vol. 4, No. 5 1449-1464
© 1977

Articles

Strandedness of newly synthesized short pieces of polyoma DNA from isolated nuclei*

P.J. Flory, Jr.**

Department of Biochemistry, Medical Nobel Institute, Karolinska Institute 104 01 Stockholm, Sweden

Received February 14, 1977.

The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured Hpajj restriction fragments. In every case an excess (1.4– to 2.4–fold) of short pieces of the strand growing in the 3'-> 5' direction was found.

*This contribution is dedicated to Jerome Vinograd.

**Present address: Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90024.


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