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Nucleic Acids Research, 1977, Vol. 4, No. 5 1483-1504
© 1977

Articles

Polyoma-induced stimulation of cellular RNA synthesis is paralleled by changed expression of the viral genome*

Consuelo Salomon, Hans Türler and Roger Weil

Department of Molecular Biology, University of Geneva 1211 Geneva 4, Switzerland

Received January 21, 1977.

We studied synthesis of viral and cellular RNA in the presence and absence of 5-fluorodeoxyuridine (FdU, an inhibitor of DNA synthesis) during lytic infection with polyoma virus in confluent, primary mouse kidney cell cultures. In the presence of FdU, synthesis of early 19S polyoma mRNA and of polyoma tumor (T)-antigen, i.e. expression of the early viral gene, is rapidly followed by a mitogenic reaction of the host cell; it leads to an increase of 30±5% in cellular, mainly 28S and 18S rRNA, followed by activation of the cellular DNA-synthesizing apparatus. Polyoma-induced cellular RNA synthesis is paralleled by increased production of early 19S mRNA and begin of expression of the late viral genes, leading to synthesis of small amounts of late 19S and 16S mRNAs. Changed expression of the viral genome occurs in the absence of detectable synthesis of polyoma DNA I. Infection in the absence of FdU induces the same sequence of events; it is followed, however, by duplication of the mouse cell chromatin (S-phase) and production of progeny virus.

*dedicated to Jerome Vinograd.


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