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Nucleic Acids Research, 1977, Vol. 4, No. 5 1539-1552
© 1977

Articles

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide{dagger}

James Casey and Norman Davidson

Department of Chemistry, California Institute of Technology Pasadena, CA 91125, USA

Received December 24, 1976.

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

{dagger} Dedicated to Jerome vinograd.


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