Skip Navigation

This Article
Right arrow Print PDF (696K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (37)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lebowitz, J.
Right arrow Articles by Kitos, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lebowitz, J.
Right arrow Articles by Kitos, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 6 1695-1712
© 1977

Articles

Carbodiimide modification of superhelical PM2 DNA: considerations regarding reaction at unpaired bases and the unwinding of superhelical DNA with chemical probes

J. Lebowitz*,*, A. K. Chaudhuri{dagger}, A. Gonennet{dagger} and G. Kitos{dagger}

*Dep. Microbiol. and Comprehensive Cancer Cent., Univ. Alabama in Birmingham Birmingham, AL 35294 {dagger}Dep. Biol., Syracuse Univ. Syracuse, NY 13210, USA

Received December 15, 1976.

Superhelical PM2 DNA I can be modified with N-cyclohexyl-N'-ß-(4-methylmorpholinium)ethyl carbodiimide (CMC). The transition of the sedimentation coefficient uncorrected for buoyant density change (S20,*) vs. % reactivity in terms of base pairs shows the following characteristics. The S20,* increases by 4.5 S units upon 1% modification. There is a plateau'in S20,* between 1 and 4% reactivity. The extent of reactivity was determines'by buoyant density and 14C radioactive CMC binding measurements. Further reactivity was not explored since Pulleyblank and Morgan's (22) data of S20,* vs. % reactivity from 6 to 34% was previously published. The initial results obtained in this study are complementary to the cited results of the above authors. Consequently, both sets of data taken together represent a complete description of S20,* vs. % reactivity with CMC. It is shown that the model in which supernalical DNA is proposed to contain small intrastrand hairpin regions can be extended to account for the observed transitions in S20,* vs.reactivity.

*Address all reprint requests to J. Lebowitz.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.