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Nucleic Acids Research, 1977, Vol. 4, No. 6 1783-1792
© 1977

Articles

A circular dichroism study of ONA-basic peptides associations in the absence or in the presence of Ca++

Catherine Lacombe and Alain Laigle

Service de biophysique C.H.U. Pitieé-Salpeêtrieère, 91 boulevard de I'Hoôpital, 75013, Paris, France

Received January 27, 1977. The interaction of DNA with basic peptides (Lys methyl ester*, Lys2, (Lys)2 methyl ester) has been studied by circular dichroism.

The changes of the DNA CD spectra in the presence of peptides are interpreted as a tjansconformation from the B form to the C form of DNA. The presence of Ca++ in the mixture induces a supplementary transconforma-tion.

These observations suggest Ca++ -basic peptides-DNA complexes as a structural model for chromatin.


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