Skip Navigation

This Article
Right arrow Print PDF (692K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (103)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Christiansen, G.
Right arrow Articles by Griffith, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christiansen, G.
Right arrow Articles by Griffith, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 6 1837-1852
© 1977

Articles

Salt and divalent cations affect the flexible nature of the natural beaded chromatin structure

Gunna Christiansen and Jack Griffith

Biochemistry Department, Stanford University Medical School Stanford, CA, USA

Received February 15, 1977. A natural chromatin containing simian virus 40 (SV40) DNA and his-tone has been used to examine changes in chromatin structure caused by various physical and chemical treatments. We find that histone HI depleted chromatin is more compact in solutions of 0.15M NaCl or 2 mM MgCl2 than in 0.01 M NaCl or 0.6M NaCl, and is compact in 0.01 M NaCl solutions if histone HI is present. Even high concentrations of urea did not alter the fundamental beaded structure, consisting of 110Å beads of 200 base pair content, each joined by thin DNA bridges of 50 base pairs. The physical bead observed by EM therefore contains more DNA than the 140 base pair "core particle". The natural variation in the bridge length is consistent with the broad bands observed after nuclease digestion of chromatin. Chromatin prepared for EM without fixation contained long 20Å to 30Å fibers possibly complexed with protein.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
K. Adolph, D. Caspar, C. Hollingshead, E. Lattman, W. Phillips, and W. Murakami
Polyoma virion and capsid crystal structures
Science, March 16, 1979; 203(4385): 1117 - 1120.
[Abstract] [PDF]

Home page
 ScienceHome page
U Muller, H Zentgraf, I Eicken, and W Keller
Higher order structure of simian virus 40 chromatin
Science, August 4, 1978; 201(4354): 406 - 415.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.