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Nucleic Acids Research, 1977, Vol. 4, No. 7 2181-2190
© 1977

Articles

A kinetic study of 1H->3H exchange in C(8) H-groups of purinic residues in DNA

G. N. Lapiashvili, E. A. Lesnik, R. N. Maslova and Ya. M. Varshavsky

Institute of Molecular Biology, Academy of Sciences of the USSR Moscow 117312, USSR

Received March 28, 1977. The kinetic study of 1H->3H exchange in C(8) of purinic residues of DNAs with different C-C content as well as in corresp dNMP mixtures have been carried out. The present results show that H--H exchange in DNA is retarded (as compared to the exchange in dNMP mixtures) to a lesser extent (Kret =2.4–2.8) than in RNA (Kret =6–8). The degree of retardation in these polymers is practically independent of their nucleotide composition. Assuming the ylide mechanism of exchange reaction it is suggested that the lower rate of 1H->3H exchange in C(18) H-groups of purinic residues in polynucleotides of A-form (RNA and other polyribonucleotides) as compared to those of B-form (DNA and other polydeoxyribonucleotides) might be accounted for by decreased availability of C(8) for OH– ions of the solvent due to a different microenviroment of these groups in A- and B-type helixes.


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