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Nucleic Acids Research, 1977, Vol. 4, No. 7 2213-2222
© 1977

Articles

DNA regions essential for the function of a bacteriophage fd promoter

T. Okamoto, K. Sugimoto, H. Sugisaki and M. Takanami

Institute for Chemical Research, Kyoto University Uji, Kyoto 611, Japan

Received April 14, 1977. The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence, TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R.Hha I cleavage site which destroys promoter function is located five base pairs upstream from the TATAAT sequence(fifteen base pairs upstream from the RNA initiation site). The promoter was cleaved into two fragments by R.Hha I and each promoter fragment was joined to DNA fragments derived from other regions. Ligation of the TATAAT-containing fragment to any of the DNA fragments examined resulted in recovery of promoter function. The results suggest for this type of promoter that no unique sequence is necessary upstream from the R.Hha I cleavage site although a contiguous DNA chain must be present in this area.


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