Skip Navigation

This Article
Right arrow Print PDF (355K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Adamiak, R. W.
Right arrow Articles by Wiewiórowski, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adamiak, R. W.
Right arrow Articles by Wiewiórowski, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 7 2321-2330
© 1977

Articles

Nucleoside 3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. IV. New method for removal of 2,2,2-trichioroethyl group and 31 NMR as a new tool for analysis of deblocking of intemucleotide phosphate protecting groups

R. W. Adamiak, E. Biala, K. Grzéskowiak, R. Kierzek, A. Kraszewski, W.T. Markiewicz, J. Stawínski and M. Wiewiórowski*;

Department of Stereochemistry of Natural Products, Institute of Organic Chemistry. Polish Academy of Sciences, Noskowskiego 12/14, 61-704 poznán, Poland

*To whom all correspondence should be addressed

Received May 2, 1977. Zinc/acetylacetone/pyridine treatment has been designed as a very efficient method for removal of 2,2,2-trichloroethyl group from phosphoesters. Internucleotide and terminal 2,2,2-trichloroethyl phosphotriesters were transformed to corresponding diesters quantitatively. Much less reactive 2,2,2-trichloroethyl phosphodiesters produced monoesters with Ca. 90% yield.

31P NMR spectroscopy has been proposed as a new tool for analysis of removal of internucleotide phosphate protecting groups - a crucial step in oligonucleotides synthesis via phosphotriester approach.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.