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Nucleic Acids Research, 1977, Vol. 4, No. 7 2389-2406
© 1977

Articles

Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene

Peter Humphries*, Madeleine Cochet1, Madeleine Andrée Krust1, Pierre Gerlinger2, Philippe Kourilsky3 and Pierre Chambon1,**

1Lab. de Génétique Moleculaire des Cellules Eukaryotes du CNRS U44 de L'INSERM et Inst. de Chimie Biologique, Fac. de Méd., 67085 Strasbourg Cedex 2Inst. d'Embryologie, Fac. de Méd. 67085 Strasbourg Cédex 3Dep. de Biologie Moléculaire, Inst. Pasteur Paris, France

**To whom reprint requests should be sent

Received May 4, 1977.

Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E.coli plasmid pCR1 using an oligodG-dC tailing procedure. The resultant hybrid plasmids, amplified by transfection of E.coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.

*Peter Humphries was supported by a long-term EMBO fellowship.

Present address: The Beatson Institute for Cancer Research Laboratory of Molecular Pathology Glasgow Scotland, G.B.


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