Skip Navigation

This Article
Right arrow Print PDF (2171K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Flavell, R. B.
Right arrow Articles by Smith, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Flavell, R. B.
Right arrow Articles by Smith, D. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 7 2429-2444
© 1977

Articles

Hyperpolymer formation during renaturation of DNA from genomes with different sequence organisation

Richard B. Flavell and Derek B. Smith

Department of Cytogenetics, Plant Breeding Institute Cambridge CB2 2LQ, UK

Received May 9, 1977. Hyperpolymer formation during the renaturation of DNAs from wheat, calf and E. coli was studied using hydroxyapatite chromatography, electron microscopy and s1 nuclease. Large hyperpolymers could not be eluted from hydroxyapatite with 0.5 M phosphate buffer at 60°C. Large proportions of wheat and E. coli DNAs were incorporated into hyperpolymers when fragments 650 nucleotides long were renatured. A much smaller proportion of calf DNA was incorporated under equivalent conditions. Greater proportions of calf DNA accumulated in hyperpolymers only when longer fragments were incubated. Electron microscopy indicated no obvious differences in the basic structures of hyperpolymers formed by the three DNAs and confirmed the quantitative differences in hyperpolymer formation found by hydroxyapatite chromatography. It is concluded that the proportions and arrangement of the repeated sequences in the chromosomes of higher organisms determine the extent of rapid hyperpolymer formation during DNA renaturation in vitro.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.