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Nucleic Acids Research, 1977, Vol. 4, No. 7 2507-2510
© 1977

Articles

A simple, rapid preparation of{alpha}-[32p]- labelled adenosine diphosphate

I. L. Cartwright and D. W. Hutchinson*

Department of Molecular Sciences, University of Warwick Coventry, CV4 7AL, UK

*To whom correspondence should be addressed

Received June 3, 1977. Hexokinase (EC 2. 7. 1. 1) will convert commercially available {alpha}-[32p]-labelled ATP into {alpha}-[32p]-labelled ADP. A simple, rapid isolation procedure for the {alpha}-[32p]-labelled ADP is described and this synthetic method can be used for the preparation of other {alpha}-[32p]-labelled nucleoside diphosphates.


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