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Nucleic Acids Research, 1977, Vol. 4, No. 8 2931-2937
© 1977

Articles

Enzymatic synthesis of Q* nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver

Norihiro Okada and Susumu Nishimura

Biology Division, National Cancer Center Research Institute Tsukiji 5-1, Chuo-ku, Tokyo, Japan

Received June 22, 1977. The Q* nucleosides isolated from rabbit liver tRNA are known to have sugars (mannose or galactose) linked to their cyclopentene diol moiety. A Q* nucleoside containing mannose (manQ) was synthesized by a cell-free system from rat liver, using purified E. coli tRNAAsp as an acceptor and GDP-mannose as a donor molecule. The novel mannosyltransferase catalyzing this reaction was purified from a particulate-free soluble enzyme fraction and found to be strictly specific for tRNAAsp. These results, together with the anomeric configuration of mannose in Q* nucleoside, indicate that no lipid intermediate is involved in the biosynthesis of Q* nucleoside.


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